ABSTRACT
Characteristic chromatogram of Ligustri Lucidi Fructus which were processed by heat treatment with different temperatures and times was developed by HPLC. DPPH, ABTS and FRAP assays were performed to determine the antioxidant activity. The spectrum-effect correlation was studied using Partial Least Squares(PLS)to explore the active ingredients of Ligustri Lucidi Fructus by investigating the relationship between HPLC spectrum and antioxidant activity. S1, S2, S15, S24, S27, S29, S32, S33 and S35 were characteristic compounds in 35 matching characteristic chromatograms which were positive relationship with scavenging DPPH free radical activity. S1, S2, S5, S8, S16, S27 and S33 were significantly positively related to scavenge ABTS free radical activity. S12, S20, S22, S28, S30, S31, S32 and S34 were significantly positively related to restore Fe³⁺ activity. Among the chromatographic peaks, S1, S2, S27 and S33were positively related to scavenge DPPH free radical and ABTS free radical activity, and S32 was positively related to scavenge DPPH free radical and restore Fe³⁺ activity. S3, S19, S21, S22, S23, S33 and S34 were determined as salidroside, luteolin-7-O-glucoside, specnuezhenide, oleuropein, ligustroflavone, luteolin andapigenin, respectively. The results showed that although specnuezhenide and salidroside were stipulated index compounds to evaluate the quality of Ligustri Lucidi Fructus, the Ligustri Lucidi Fructus pharmcodynamic effect did not depend on the contents of those index components. The quality of traditional Chinese medicines(TCMs)should be determined by the compound groups associated with pharmcodynamic effect.
ABSTRACT
Consult literature, the chemical composition of the type of system Caesalpinia plants were summarized and discussed in detail its pharmacological effects. The genus contains major chemical components of high isoflavones, chalcones and diterpenoids, pharmacological effects inhibit melanin production, anti-inflammatory, antioxidant, antibacterial, immune regulation, and further research in this genus offer reference.
ABSTRACT
To study the heating treatment of Angelica dahurica under different temperature and time conditions on the stability of coumarins and tyrosinase activity. HPLC method was used to determine the contents of imperatorin and isoimperatorin, and tyrosinase activity was assayed by measuring the oxidation rate of L-DOPA in vitro. After heated, the contents of imperatorin increased. Expect for being heated at 90 ℃ for 2 h, the content of isoimperatorin was higher than crude one. Before and after being heated, A. dahurica showed an activating effect on tyrosinase. In the same temperature and time conditions, the activation rate increased with the rise of concentration of tyrosinase extracts. Heating process for A. dahurica could change the contents of imperatorin and isoimperatorin, mainly increasing their concentrations.
ABSTRACT
OBJECTIVE: To study the antimicrobial activity and antibacterial mechanisms of cryptotanshinone against SA, MRSA and ESBLS-SA. METHODS: MIC value was determined by disc diffusion method. IC50 value was determined by liquid culture. The antibacterial mechanisms of cryptotanshinone were investigated by determining the changes in electric conductivity, concentration of AKP, protein content, and the changes of protein electrophoretic bands in SDS-PAGE. RESULTS: The antibacterial rings, MIC and IC50 values showed that the antimicrobial activity against SA was better than that against MRSA and ESBLs-SA. Treated with cryptotanshinone, the electric conductivity, concentration of AKP, and protein content were increased, and protein bands in SDS-PAGE were changed obviously. CONCLUSION: Cryptotanshinone significantly inhibits SA, MRSA and ESBLs-SA and can damage the structures of cell wall and cell membrane, which results in the increase of permeability of cell membrane and release of cell components. Cryptotanshinone can influence the synthesis of bacteria protein, destroy the protein or reject the anabolism or expression of the protein, and finally leads to the loss of normal physiological function of bacterium.
ABSTRACT
<p><b>OBJECTIVE</b>To study the validity of Schizonepeta tenuifalia pieces.</p><p><b>METHOD</b>The change principle for the contents of essential oil and pulegone was determined by the way of classical constant temperature acceleration experiment, and the reacting speed at 20 degrees C was calculated according to Arrhenius index law and effective time was calculated.</p><p><b>RESULT</b>In classical constant temperature experiment, the content change of essential oil with the regular pattern of one level. The effective time of S. tenuifalia pieces stored in 20 degrees C was 2.08 years and the constant of speed is 8.453 4 x 10(-6) carried out by essentialoil, 190 days with the constant of speed is 3.39 x 10(-5) carried out by pulegone.</p><p><b>CONCLUSION</b>The effective time of S. tenuifalia pieces was about 2 years carried out by essential oil and 190 days carried out by pulegone by the way of classical constant temperature experiment.</p>
Subject(s)
Drug Stability , Lamiaceae , Chemistry , Monoterpenes , Oils, Volatile , Temperature , Time FactorsABSTRACT
<p><b>OBJECTIVE</b>To predict the stable life for Mentha haplocalyx.</p><p><b>METHOD</b>The volatiles in M. haplocalyx were analyzed by head-space solid micro-extraction, coupled with GC-MS and a comprehensive evaluation of essential oil in M. haplocalyx was analyzed using the factor analysis. The prediction was carried out by initial average rate stability tests using the content of essential oil and the main volatiles as indices.</p><p><b>RESULT</b>Principal component analysis indicated that pulegone and isomenthone can fully describe the quality of prepared slices. The t(0.9, 20 degrees C) was 5.49 years and 2.88 years respectively, carried out by essential oil, pulegone and isomenthone.</p><p><b>CONCLUSION</b>The stable life for M. haplocalyx under 20 degrees C was 2.88 years.</p>